Barley grain was sprouted in a still hydroponic growing chamber for 6, 7 and 8 day periods and sampled for chemical analyses, protein fractions,in vitro digestion and metabolisable energy (ME) determination. Productivity measured on the basis of the input-output balance of barley grain and GF yield. Results showed that CP, Ash, EE, NDF, ADF and water soluble carbohydrate (WSC) were increased whereas OM and non fiber carbohydrate (NFC) decreased (p < 0.05) in the GF when compared with the original grain. As the growing period extended from day 6 to day 8, the CP, Ash, EE, NDF and ADF were increased but NFC and WSC reduced (p < 0.05). The non protein nitrogen was increased but true protein decreased (p < 0.05) in GF in comparison to barley grain, however no differences was shown among the growing periods for protein fractions. The potential (b) and rate (c) of in vitro gas production shown a decreasing trend (p < 0.05) by sprouting the barley grain up to 8 days. The amount of OM and ME of GF, obtained per kg of cultivated barley grain, were lower than those of the original grain.
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