The effect of four enzymes: collagenase, fibrinolysin, hyalurodinase, and trypsin were recorded on the viscosity, motility,percent live spermatozoa and acrosome integrity of Llama and Alpaca semen. Semen samples were collected using a modified artificial vagina for each of the five llamas and five alpacas. A 25% solution of the of enzyme at a concentration of 1 mg/ml was added to the ejaculate. Analysis of variance was used to determine differences in eliminating viscosity and alterations in motility, percent live spermatozoa and the acrosomal integrity at 0 (time of semen collection), 2 and 5 min. In Llama and Alpaca semen, collagenase eliminated viscosity in 100 and 99% of the samples, respectively. Correspondingly, fibrinolysin in 89 and 59%; hyalurodinase in 88 and 36%; and trypsin in 55 and 68% of the samples (p
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